Claudin-1 and ALT were negatively correlated with 13-hydroxy-alpha-tocopherol, docosatrienoic acid, oleanolic acid, pentadecanoic acid, L-(+)-aspartic acid, 4-phenylbutyric acid, and 2-arachidonoylglycerol. Effect of supplementing fermented milk on pathological changes of liver, adipose tissue and colon tissue in mice: (AC) H&E of liver tissue sections, epididymal white adipose tissue (epididymal WAT), brown fat (BAT), (D,E) glutamic pyruvic transaminase (ALT) and glutamic oxalacetic transaminase (AST), (F) leptin. Fig. P.R., B.L.R., C.S., T.G.D. The extract or supernatant may be in any physical form, for example liquid or dried. DOI: https://doi.org/10.1016/j.ebiom.2020.103176, We use cookies to help provide and enhance our service and tailor content and ads. FIG. Impaired intestinal barrier integrity is considered to be an important cause of various systemic metabolic diseases such as insulin resistance, obesity, and type 2 diabetes [46,47,48,49]. 19. Probiotic or synbiotic alters the gut microbiota and metabolism in a randomised controlled trial of weight management in overweight adults. Human fecal strains are derived from the previous isolation of the research group which received ethical approval [23]. Fifty mg of mouse feces was placed in a centrifuge tube. [5] Up to 19 types of permease exist to transport various carbohydrates with 13 being ATP-binding cassette transporters. Shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents. This substance is also considered biomarkers for patients with type 2 diabetic atherosclerosis [68]. Therefore, we can intervene in the treatment of certain diseases through food. Samples for the analysis of active ghrelin were immediately treated with AEBSF (final concentration 1mg/mL, Sigma, A8456), centrifuged and the resulting plasma was treated with HCl (final concentration 0.05N). Lei L., Liu Q., Liu S., Huan Y., Sun S., Chen Z., Li L., Feng Z., Li Y., Shen Z. Antidiabetic potential of a novel dual-target activator of glucokinase and peroxisome proliferator activated receptor-gamma. Perfect for breakfast or an indulgent snack without the guilt. The invention relates to an isolated Bifidobacterium longum APC1472 strain as deposited with the National Collection of Industrial and Marine Bacteria under the Accession No. Other aspects and preferred embodiments of the invention are defined and described in the other claims set out below. Manzanarez-Quin C.G., Beltran-Barrientos L.M., Hernandez-Mendoza A., Gonzalez-Cordova A.F., Vallejo-Cordoba B. The abundances of norank_f__norank_o__Rhodospirillales, norank_f__Peptococcaceae, and norank_f__UCG-010 were negatively correlated with AUC, insulin, HOMA-IR, LDL-C, TC, AST, ALT, and leptin, and positively correlated with claudin-1. Fig. The function of this is not clear, although B. longum could use the amino acids products to better tolerate bile salts. A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans. Where the composition is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients may be mixed prior to administration. The invention also provides a method of producing an extract from an isolated Bifidobacterium longum APC1472 strain comprising a step of lysing the cell and separating the cell extract from lysed cell material. BLFM supplementation significantly reduced 3-indoxyl sulphate and 4-hydroxybutyric acid levels while increasing oleanolic acid, pentadecanoic acid, and L- (+)-aspartic acid. At the end of the 13th week, the mice were fasted overnight, the weight recorded, anesthetized with carbon dioxide, the eyeballs removed for blood collection, and the mice were immediately killed by cervical dislocation. will also be available for a limited time. The weight reduction effect is achieved through increased pAMPk activity, indicating induction of metabolic effects on adipose and skeletal muscle tissue. M.vdW. Bifidobacterium longum research reveals benefits in metabolic health in areas such as obesity, liver disease, and kidney disease. Real-time PCR was performed using TaqMan Universal Master Mix II, no Uracil-N glycoslyase (UNG) on a LightCycler480 System (Roche). 12. What is Bifidobacterium longum? doi: 10.1128/msystems.00343-22. WO2011/039176 describes a strain of Bifidobacterium longum BL999 capable of reducing weight gain in a high fat diet fed mouse over a seven week period. A toxicologists guide to biomarkers of hepatic response. Reviews in endocrine & metabolic disorders. Briefly, data from the 30-, 45- and 60-minute time-points were normalized (delta) to the samples taken immediately upon awakening, after which the sum was taken of the 30-, 45- and 60-minute time-points [, Murine caecal DNA was isolated using the QIAamp Fast DNA Stool Mini kit (Qiagen) as previously described and kept at 20C until further analysis [, For the human intervention study, faecal sample collection and DNA extraction was performed as previously described (see supplementary material for details) [. Water intake was monitored throughout the experiment. [2] These bacteria alleviate glucose and lipid metabolism disorders caused by obesity and a high-fat diet [56,57,58,59,60] by the production of succinic acid and secondary bile acids. 2015 Jan;77(1-2):229-35. doi: 10.1038/pr.2014.156. The daily consumption (2.9 106 CFU/day) of B. longum subps. It is subspecies B. longum subsp. FLASH: Fast length adjustment of short reads to improve genome assemblies. Infantis isolates", "Phenotypic and genotypic characterization of Bifidobacterium isolates from healthy adult Koreans", "A Proteome Reference Map and Proteomic Analysis of Bifidobacterium longum NCC2705", "Bifidobacterium longum as a delivery system for cancer gene therapy: Selective localization and growth in hypoxic tumors", "Proposal to reclassify the three biotypes of Bifidobacterium longum as three subspecies: Bifidobacterium longum subsp. Data in table are presented as mean SEM or 95% CI. Food intake was recorded once per week and calculated on the basis of two mice per cage and five cages per group. mSystems. 2. No. Trienoic acid, oleanolic acid, 13-hydroxy--tocopherol, and L-(+)-aspartic acid production significantly reduced GHB and 3-indoxyl sulphate and repaired the damaged intestinal barrier. Leptin levels in human and rodent: Measurement of plasma leptin and. It is an object of the invention to overcome at least one of the above-referenced problems. Pharmaceutical compositions formulated or configured for oral administration and gastric transit are known in the art and include those described below: As used herein, the term food refers to a man-made food product including beverages, food additives and food supplements. The HFHS group had 4250 kcal/kg and the energy supply ratio was 16.46% protein, 45.65% fat, and 37.85% carbohydrate. A pharmaceutical composition according to claim 10, configured for targeted release in the intestine. [4] FM modulates the gut microbiota: (A) Shannon index, (BD) Plots of PCoA, NMDS, and PCA scores based on the Bray-Curties distance algorithm, (E) Relative abundance of gut microbiota at the phylum level, (F) Ratio of Firmicutes to Bacteroidetes, (G) Relative abundance of gut microbiota at the genus level, (H) Relative abundance of bacterial communities at the genus level among different groups, (I) H&E of colon tissue sections, (J) The content of Claudin-1 in the colon, (K) The content of ZO-1 in the colon. 2B). Prebiotic refers to a material or composition that can promote the growth of probiotic microbes or bacteria, especially bacterial growth in the mammalian gastrointestinal tract. Table S2: Differential metabolites between BLFM and HFHS groups. An isolated Bifidobacterium longum APC1472 strain of claim 1 in a dried or lyophilised form. Ghrelin at the interface of obesity and reward. Ghrelin signalling and obesity: at the interface of stress, mood and food reward. Longitudinal Changes in Liver Aminotransferases Predict Metabolic Syndrome in Chinese Patients with Nonviral Hepatitis. Blend of 10 Strains of Probiotic Bacteria (25 Billion CFU): Lactobacillus acidophilus (La-14), Bifidobacterium lactis (Bl-04), Lactobacillus plantarum (Lp-115), Lactobacillus casei (Lc-11), Lactobacillus rhamnosus (Lr-32), Lactobacillus paracasei (Lpc-37), Bifidobacterium breve (Bb-03), Streptococcus thermophilus (St-21 . Supports gut health . Pediatr Res. B. longum APC1472 treatment completely normalized glucose levels after 15 of glucose administration in HFD-fed mice (FIG. configured for gastric transit and ileal release). Cecal DNA was isolated using the QIAamp Fast DNA Stool Mini kit (Quiagen) accordingly as previously described and kept at 20 C. until further analysis (Burokas et al 2017). Bifidobacterium Longum decreases the level of histamine. In another recombination strategy, yeast-derived FLP recombinase may be utilized with the consensus sequence FRT (see also, e.g. * indicates significant diet treatment effect (p0.05) and # indicates significant B. Longum APC1472 treatment effect. Top Probiotic Supplements: Quick Look 1. All experimental involving human participants were reviewed and approved by Ethics Committee of The First Affiliated Hospital of Guangdong Pharmaceutical University (reference 2017-98). A method of treating or preventing obesity, inducing satiety, or reducing weight gain in a subject, comprising a step of administering a therapeutically effective amount of a composition according to claim 3 to the subject. Informed consent was obtained from all subjects involved in the study. Alihosseini N., Moahboob S.A., Farrin N., Mobasseri M., Taghizadeh A., Ostadrahimi A.R. Treatment of glucose intolerance may also include dietary or lifestyle changes, including weight loss, increased level of exercise, and possibly also pharmaceutical intervention. The Deposit was made by the APC Microbiome Institute, an Institute forming part of University College Cork, National University of Ireland, Cork, and having an address of UCC, Cork, Ireland. The present invention relates to an isolated strain of Bifidobacterium longum, compositions comprising the strain, and uses of the strain or composition to treat obesity and manage weight in a subject. As probiotic activity varies among strains of B. longum, interest exists in the exact classification of new strains, although this is made difficult by the high gene similarity between the three biotypes. All experiments were approved by the Animal Experimentation Ethics Committee at University College Cork and carried out in accordance with the relevant guidelines200 European Directive 2010/63/EU. This was performed at the end of the 12th week of the experiment. The invention also relates to a method of reducing weight gain in a subject, typically a subject in need thereof, comprising a step of administering a therapeutically effective amount of bacterium or composition of the invention to the subject. Which foods contain Bifidobacterium longum? Methods and results: BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. A single colony from each strain was inoculated in MRS broth medium and incubated overnight under anaerobic conditions at 37 C. A subculture was then carried out by adjusting the OD600 to 0.05 and incubated overnight under anaerobic conditions at 37 C. for 14 hours approx. Nucleic acids may also comprise a regulatable inducible promoter (inducible, repressable, developmentally regulated) as opposed to a constitutive promoter such as CMV IE (the skilled artisan will recognize that such terms are actually descriptors of a degree of gene expression under certain conditions). However, studies have shown that it can stimulate the secretion of human glucagon in contrast to impaired regulation of glucagon secretion in type 2 diabetes patients with hyperglucagonemia [69]. In one embodiment, it refers to a cessation of weight gain (i.e. Can Bifidobacterium cause weight gain? Examples of foods include dairy products such as milk, yoghurt, cheese, cheese food, dairy powders, probiotic formulations, infant formula powders, follow-on milk formula, food for special medicinal purposes, meat products, soups, vegetable products, fruit juices, fruit products, breads, confectionary, cakes, sports supplements, nutritional supplements and the like. The insulin resistance index (HOMA-IR) was expressed as fasting blood glucose (mmol/L) fasting insulin (mIU/mL)/22.5. Vol 2 ASM Press, Washington D.C. 1996). The invention also provides a supernatant or bacterial material or extract (for example a cell lysate) formed according to the method of the invention. Learn more Hek-GHSR1a-EGFP cells were seeded in a 96-well microtiter plate at 3104 cells/well and incubated for 48 hours in Dulbecco's Modified Eagle's Medium-high glucose (DMEM) (Sigma-Aldrich, Ireland) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich) and 1% non-essential amino acids (Gibco) at culture conditions (5% CO2-humidified atmosphere at 37 C.). Would you like email updates of new search results? NCIMB 42795 on 1 Aug. 2017. In the context of treatment and effective amounts as defined above, the term subject (which is to be read to include individual, animal, patient or mammal where context permits) defines any subject, particularly a mammalian subject, for whom treatment is indicated. Cai W., Xu J., Li G., Liu T., Guo X., Wang H., Luo L. Ethanol extract of propolis prevents high-fat diet-induced insulin resistance and obesity in association with modulation of gut microbiota in mice. A composition according to claim 3, comprising a probiotic material or a prebiotic material. The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/nu14194050/s1, Figure S1: SDS-PAGE of G6PDH and GK. The HFHS group had severe glucose intolerance and dyslipidemia. 2, white panel) was lower in treated mice as compared with control . PCR products were quantified with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) and separated using 2% agarose gel electrophoresis. The pH was adjusted to 7.2 with 1 moL/L NaOH solution and centrifuged at 12,000 g for 10 min at 4 C. Glucose tolerance was assessed after 15 weeks treatment accordingly to Suez et al., (Suez, Korem et al. In an exemplary cell expression system, cells are engineered to express the tetracycline repressor protein (TetR) and a protein of interest is placed under transcriptional control of a promoter whose activity is regulated by TetR. Liu Q., Yu Z., Tian F., Zhao J., Zhang H., Zhai Q., Chen W. Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier. Commonly, selectable marker genes express proteins, usually enzymes, that confer resistance to various antibiotics in cell culture. eCollection 2022. The carrier may be configured for controlled release in the intestine (i.e. TABLE 1 Antibiotic Susceptibility Profile of Bifidobacterium longum APC1472 strain Strain Gm Sm Tc Em Cl Cm Am Va B. longum 16- 64- 1 0.06- 0.06- 2 2 0.5 APC1472 64 128 0.12 0.12 Gm: Gentamicin, Sm: Streptomycin, Tc: Tetracycline, Em: Erythromycin, Cl: Clindamycin, Cm: Chloramphenicol, Am: Ampicillin, Va: Vancomycin MICs (in g/mL) for Bifidobacterium longum ssp. The .gov means its official. nov", "Effects of Milk Products Fermented by Bifidobacterium longum on Blood Lipids in Rats and Healthy Adult Male Volunteers", "Bile salt hydrolase of Bifidobacterium longum-biochemical and genetic characterization", "Subspecies Bacteremia in 3 Extremely Preterm Infants Receiving Probiotics", "Bifidobacterium longum Bacteremia in Preterm Infants Receiving Probiotics", "Case of Sepsis Caused by Bifidobacterium longum", "Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease", "Efficacy of an Encapsulated Probiotic Bifidobacterium infantis 35624 in Women with Irritable Bowel Syndrome", New Strain of Bifidobacterium May Help Improve Metabolic and Mental Health, "Bifidobacterium longum 1714 Strain Modulates Brain Activity of Healthy Volunteers During Social Stress", https://en.wikipedia.org/w/index.php?title=Bifidobacterium_longum&oldid=1104452471, This page was last edited on 15 August 2022, at 02:51. Then, 200 L of extract (2:2:1 methanol:acetonitrile:water) was added, vortexed for 120 s, sonicated at 4 C for 20 min, incubated at 20 C for 1 h, and centrifuged at 13,800 g for 15 min at 4 C. 2022 Oct 26;7(5):e0034322. PMC The weight reduction effect is achieved through increased pAMPk activity, indicating induction of metabolic effects on adipose and skeletal muscle tissue. ELISA showed that colonic ZO-1 and Claudin-1 levels were significantly lower in the HFHS group than those in the control group, indicating that the integrity of the intestinal mucosal barrier was destroyed with a HFHS diet. Zandani G., Anavi-Cohen S., Tsybina-Shimshilashvili N., Sela N., Nyska A., Madar Z. In one embodiment, the inflammation is gut inflammation associated with an inflammatory disorder of the gut, for example Inflammatory Bowel Disease or a similar condition. Therefore, BLFM intervention significantly improved mucosal permeability and restored the integrity of the intestinal mucosal barrier by regulating tight junction proteins. Roman Chamomile. Probiotic aliquots were freshly prepared every week. The inclusion criteria were as follows: subjects had to give written informed consent; had to be between 18 and 65 years of age; had a BMI between 28 and 34.9; had a W/H ratio 0.88 for males and 0.83 for females; had to be willing to consume the investigational product daily for the duration of the study. Sternisha S.M., Miller B.G. For example, the intake of navy beans and broccoli reduced the F/B ratio [9,52], and our supplementation with BLFM similarly reduced the F/B ratio compared. 2022 Jan 5;9:795970. doi: 10.3389/fped.2021.795970. This study has a parallel-controlled design. 10. 3. Increased oral detection, but decreased intestinal signaling for fats in mice lacking gut microbiota. A Cre-lox strategy requires at least two components: 1) Cre recombinase, an enzyme that catalyzes recombination between two loxP sites; and 2) loxP sites (e.g. Milk polar lipids modulate lipid metabolism, gut permeability, and systemic inflammation in high-fat-fed C57BL/6J ob/ob mice, a model of severe obesity. As such, the terms a (or an), one or more, and at least one are used interchangeably herein. Insertion of the nucleic acid into the heterologous host is performed by recombinant DNA technology. BLFM regulated the gut microbiota, increased F/B, significantly increased the abundance of Bifidobacteria, and promoted the production of pentadecanoic acid, 4-phenylbutyric acid, and docosatrienoic acid in mice with metabolic disorders induced by diet. Amacher D.E. As used herein, the term pharmaceutical composition refers to a therapeutically effective amount of the strain of the invention, and a pharmaceutically acceptable carrier. In certain embodiments, the vector comprises one or more selectable marker genes selected from the group consisting of bla, bls, BSD, bsr, Sh ble, hpt, tetR, tetM, npt, kanR and pac. For the human intervention study, differences between the treatment and placebo groups at the last visit (i.e. Technol. 5). Yu Q., Lai B.K., Ahooghalandari P., Helander A., Gylfe E., Gilon P., Tengholm A. Gamma-hydroxybutyrate does not mediate glucose inhibition of glucagon secretion. Trunk blood was collected in tubes containing 25M dipeptidyl peptidase IV (DPP-IV) inhibitor, 2x protease inhibitor cocktail (Roche) (diluted in PBS) and 0.1% Na. This study explored the benefits and underlying mechanisms of Bifidobacterium longum 070103 fermented milk (BLFM) in thirteen-week high-fat and high-sugar (HFHS) fed mice using omics techniques. The effect of BLFM supplementation on glucose and lipid metabolism disorders in HFHS fed mice was tested by analyzing the relevant markers. The abundances of UBA1819, Lachnospiraceae_UCG-001, and Colidextribacter were negatively correlated with insulin, HOMA-IR, LDL-C, TC, AST, and ALT. Know the uses, side effects, composition, precautions and more about Juxtapro Capsule 10's. (A) Weekly body weight gain, (B) total body weight gain and (C) mesenteric, (D) retroperitoneal, (E) subcutaneous and (F) epididymal fat depots accumulation (% of total body weight) in control mice treated with drinking water containing sterile PBS (2% v/v) and glycerol (0.5% v/v) and fed a control low-fat diet (LFD) (. Overall, probiotics play a beneficial role in gut health. Moreover, the strain also showed a trend towards decreased ghrelin receptor hypothalamic expression in HFD-fed mice when compared with vehicle control group. A single-blind, placebo-controlled trial (SYNERGIE) was conducted in 2- to 6-month-old Bangladeshi infants with SAM. The assay was performed according to the manufacturer's protocol with some modifications. B. longum APC1472 treatment trended to restore the HFD-associated decrease in Bifidobacterium at the family level (FIG. It comprises about 90% of all forms of diabetes, the other forms being Type 1 diabetes and gestational diabetes. sharing sensitive information, make sure youre on a federal longum FA1 Reduced Weight Gain and Hepatic Lipid Droplets in Young Mice Fed High-Fat Mustafa Alsharafani 1 *, Martin R In this study, Spearmans correlation analysis was used to determine the degree of correlation between changes in gut microbiota following supplementation with fermented milk, and host metabolic parameters. Santaren I.D., Watkins S.M., Liese A.D., Wagenknecht L.E., Rewers M.J., Haffner S.M., Lorenzo C., Hanley A.J. Resistance to diet-induced obesity is associated with increased proopiomelanocortin mRNA and decreased neuropeptide Y mRNA in the hypothalamus. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions. A cell extract or supernatant of Bfidobacterium longum APC1472 strain. This partially regulates the gut microbiota, improves the intestinal barrier, and alleviates glucose and lipid metabolism disorders caused by HFHS. The term also includes mutants and variants of the deposited strain that are substantially identical, genetically and phenotypically, to the deposited strain and retain the activity of the deposited strain. [13], A number of cases of B. longum infection have been reported in the scientific literature. nov. and Bifidobacterium longum subsp. Data are significant different (p0.05) accordingly to Repeated Measures ANOVA (A) or two-way ANOVA followed by LSD post-hoc test (B, C, D, E and F). 1College of Food Science, South China Agricultural University, Guangzhou 510642, China, 2Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China. Table S1: Screening of glucokinase activator in vitro (77 samples). The mice were fed a low-fat diet (LFD) (10% fat (kcal/100 g), D12450B, Research Diet, USA) or fed a high-fat diet (HFD) (45% fat (kcal/100 g), D12451, Research Diet, USA) for 16 weeks. Chen Q.C., Xiao J., Zhang P.P., Chen L.L., Chen X.X., Wang S.M. The content on this site is intended for healthcare professionals. After sequencing, reads were assembled, processed and analyzed as previously described (Burokas, Arboleya et al. Blood samples for the analysis using the U-PLEX assays were treated with DPP-IV inhibitor (final concentration 1%, Sigma, DPP4) and centrifuged. 3G). Compositions of the invention generally comprise between 103 and 1012 cfu of the strain of the invention per gram of dry weight of the composition. The carrier may be in the form of tablet, capsule, powder, granules, microparticles or nanoparticles. Circulating ghrelin levels are decreased in human obesity. Subjects were removed from the study if they independently elected to withdraw; he/she developed any condition which contravened the original criteria; or was considered at any point to be unsuitable to continue the study, at the discretion of the investigator. To monitor the cortisol awakening response, saliva from visits 2 and 4 was collected in Salivette devices (Sarstedt, 51.1534.500) immediately upon awakening, and after 30, 45 and 60min. Studies also show that consumption of B. Longum and other probiotics can help decrease the allergic effect by suppressing histamine signaling. official website and that any information you provide is encrypted However, not significant B. longum APC1472 treatment effects were found for fasting insulin levels (FIG. Values with superscript letters a, b and c are significantly different across rows (p < 0.05). A natural solution for obesity: bioactives for the prevention and treatment of weight gain. Bifidobacterium can be found plentifully in many food types including: goat dairy products, like yogurt, kefir, seaweed, and miso soup [14]. A therapeutic result in this context includes eradication or lessening of symptoms, reduced pain or discomfort, prolonged survival, improved mobility and other markers of clinical improvement. BMC Pediatr. Same story, different bacteria! 5. (See, e.g., U.S. Pat. The authors would like to thank Lorenzo Miorelli from Sacco (Sacco SRL, Italy) for coordinating, Accepted: However, there is a need for systematic research on the improvement of glucose and lipid metabolism parameters and the gut microbiota and its metabolites. Where necessary, the composition may also include a solubilizing agent and a local anesthetic such as lignocaine to, ease pain at the, site of the injection. 18. MAJORITY OF AMERICANS ADMIT TO WEIGHT GAIN DURING CORONAVIRUS PANDEMIC, PLAN TO SHED POUNDS IN 2021 . The bacterium significantly decreased levels of leptin and significantly reduced the level of intemalisation of the hunger hormone ghrelin receptor, indicating that the anti-obesity effect is at least partially mediated through increased satiety in a subject. Plates were incubated at 37 C. for 48 h. After 48 h the plates were read visually. Serum pentadecanoic acid (15:0), a short-term marker of dairy food intake, is inversely associated with incident type 2 diabetes and its underlying disorders. It is classified in the phylum (group) Actinobacteria as a Gram-positive bacterium. DNA amplification reaction conditions were denaturation at 95 C for 120 s, followed by 45 cycles of denaturation at 95 C for 20 s, and annealing at 60 C for 60 s. The data were analyzed using the built-in software. The investigational product arrived on site labelled with randomisation number. The antibiotic susceptibility profile of the Deposited Strain is provided in Table 1 below. Obesity: current and potential pharmacotherapeutics and targets. In one study, a strain of Bifidobacterium longum exhibited a more significant effect in lowering serum total cholesterol than a mixed culture of Streptococcus thermophilus and . Asemi Z., Zare Z., Shakeri H., Sabihi S.S., Esmaillzadeh A. However, mice supplemented with LGGFM and BLFM showed a decreasing trend compared with HFHS, and BLFM significantly reduced the BW (Figure 1B). a specific 34-base pair by sequence consisting of an 8-bp core sequence, where recombination takes place, and two flanking 13-bp inverted repeats) or mutant lox sites. government site. Assessment of the cortisol awakening response: expert consensus guidelines. Selective Isolation of Bifidobacterium from Human Faeces Using Pangenomics, Metagenomics, and Enzymology. 2014) with modifications. Supplementation with LGGFM and BLFM significantly reduced leptin resistance caused by obesity in mice fed a HFHS diet (Figure 3F). Here we found that a 45% fat (kcal/100 g) diet led to a significant increased body weight after 2 weeks of treatment (FIG. HHS Vulnerability Disclosure, Help Data are normalized to the maximum expected receptor internalization obtained upon treatment with the agonist ghrelin, analyzed and depicted using GraphPad Prism software (PRISM 5.0; GraphPAD Software Inc., USA). In one embodiment, the method is a method of reducing total plasma cholesterol, triglyceride, or low density lipoprotein (LDL) levels, or increasing total high density lipoprotein (HDL) levels, in the subject. The new PMC design is here! All fecal DNA of BLFM was extracted for qPCR analysis in triplicate. Current pharmacologic anti-obesity treatments lack efficacy and have shown severe side effects, highlighting the urgent need for novel strategies contributing to the maintenance of a healthy weight. The invention also relates to a supernatant or cell material derived from the isolated APC1472 strain. An official website of the United States government. Such pharmaceutical carriers can be sterile liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. At the same time, the feces of mice fed with BLFM were evaluated to detect the colonization ability of B. longum, and the number was 4.36 0.013 lg/g of feces (Supplementary Figure S2). M.C., Y.D., J.Z., J.W., X.X. The metabolites with significant differences from the control were further analyzed for pathway enrichment analysis and KEGG analysis to determine the influence of consuming probiotic fermented milk on the fecal metabolites of mice on a HFHS diet using the model organism mice as a reference. The strain also improved glucose tolerance and decreased insulin plasma levels in mice, indicating the strain in the prevention and/or treatment of Type II diabetes, especially in obese individuals. Dissected mouse liver, epididymal fat, brown fat, and colon tissue were fixed with 10% formalin solution, embedded in paraffin, sliced into 3-mm thick sections using a semi-automatic paraffin microtome (Thermo Fisher Scientific, Waltham, MA, USA), and stained with H & E. Pathological changes were observed with a digital biological microscope (Nanjing Jiangnan Novel Optics Co., Ltd., Nanjing, China) using ScopeImage 9.0 software (Ningbo Yongxin Optics Co., Ltd., Ningbo, China). Symptoms of glucose intolerance include thirst, dry mouth, extreme tiredness, blurred vision, drowsiness, frequent need to urinate, and loss of muscle mass. Different probiotic strains have been shown to have different beneficial anti-obesity effects such as reduced body weight gain, improvements in insulin sensitivity and glucose uptake, and reduced fat depots accumulation in rodents. BLFM reverses HFHS-induced reduction in bifidobacteria abundance. Level of significant in all analysis was =0.05 and all tests were two-tailed test. Japanese cedar pollen allergy. lactis 420 may . Probiotics, pills filled with millions, sometimes billions, of healthy gut microbes are another mode of acquiring . 07/14/2022 6:14pm Collagen is a protein that acts as the "glue" of the body, literally holding everything together. Obese or Obesity as applied to an adult human means a BMI of 30 or greater. Analysis of the faecal microbiota in the human intervention study revealed that, Short-chain fatty acids (SCFAs) are potentially one of the most investigated gut microbiota-derived metabolites implicated in host energy metabolism and obesity symptomatology [. Data are presented as means SD (n = 10) and analyzed using one-way ANOVA (* p < 0.05, **** p < 0.0001). Gut microbiota composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels. Diet and exercise in management of obesity and overweight. the administration of an agent to a subject) which cures, ameliorates or lessens the symptoms of a disease or removes (or lessens the impact of) its cause(s) (for example, the reduction in accumulation of pathological levels of lysosomal enzymes). Thaiss C.A., Levy M., Grosheva I., Zheng D., Soffer E., Blacher E., Braverman S., Tengeler A.C., Barak O., Elazar M., et al. Duplicates with 20% coefficient of variability were re-analysed. Park S.E., Kwon S.J., Cho K.M., Seo S.H., Kim E.J., Unno T., Bok S.H., Park D.H., Son H.S. longum 35624, previously classified as Bifidobacterium longum ssp. Using self-report scales, participants were assessed for perceived stress using Cohen's Perceived Stress Scale and anxiety and depression using the Hospital Anxiety and Depression Scale (HADS) at baseline, after 6 and after 12 weeks, as previously described[. Chloramphenicol was used for selection of the plasmid at a concentration of 25 g/mL in both E. coli and B. longum. An isolated Bifidobacterium longum APC1472 strain as deposited with the National Collection of Industrial and Marine Bacteria under the Accession No. Bisanz J.E., Upadhyay V., Turnbaugh J.A., Ly K., Turnbaugh P.J. Effects of probiotic yogurt on fat distribution and gene expression of proinflammatory factors in peripheral blood mononuclear cells in overweight and obese people with or without weight-loss diet. longum APC1472, for antibiotics with published EFSA cut-off values. M.M.P. Standards were included in each run to maintain the calibration. Effects of supplementing fermented milk on BW and weight gain: (A) BW change in mice at 13 weeks, (B) BW gain. [17][18][19] Infection in preterm infants manifests as bacteremia or necrotizing enterocolitis,[20] while in adults there have been reports of sepsis and peritonitis. Hypothalamus was dissected with a forceps (macropunch) from the frozen brain on dry ice and immediately processed for RNA extraction. Embodiments include beta-lactamase (bla) (beta-lactam antibiotic resistance or ampicillin resistance gene or ampR), bls (blasticidin resistance acetyl transferase gene), bsd (blasticidin-S deaminase resistance gene), bsr (blasticidin-S resistance gene), Sh ble (Zeocin resistance gene), hygromycin phosphotransferase (hpt) (hygromycin resistance gene), tetM (tetracycline resistance gene or tetR), neomycin phosphotransferase II (npt) (neomycin resistance gene or neoR), kanR (kanamycin resistance gene), and pac (puromycin resistance gene). -findings from metabolome analysis using GC/MS. None of the others bacterial strains supematants investigated showed any effect. In vivo data analysis was performed with a two-way ANOVA followed by LSD post hoc test (normal distributed data) or non-parametric multiple comparisons Kruskal-Wallis test with Bonferroni adjustment of p-values (non-normal distributed data). Supplementation with fermented milk significantly increased the abundance of norank_f_Muribaculaceae, Bifidobacterium, Alloprevotella, norank_f__Oscillospiraceae, Alistipes, Bacteroides, and Parabacteroides. One hundred of cell suspension was aliquoted into 96-well plates within 30 min with a multichannel pipette to give 3104 CFU/well. Accessibility All other authors report no conflicts of interest. Data are presented as means SD (n = 10) and analyzed using one-way ANOVA (** p < 0.01, *** p < 0.001, **** p < 0.0001). Preferably, the strain or composition is administered several times a week, and ideally once a day. Secondly, probiotic strains isolated from the feces of healthy individuals were used to ferment milk. so Mann Whitney test) (p=0.077) According to a first aspect of the present invention, there is provided an isolated Bifidobacterium longum APC1472 strain as deposited with the National Collection of Industrial and Marine Bacteria under the Accession No. This shows that BLFM supplementation reduces liver damage caused by HFHS. C.M.L.S. 1. Accessibility Ghrelin (0.5 M) (Innovagen, Sweeden) was used as positive control. Body weight was weekly monitored for 15 weeks. Glucokinase activator SHP289-04 significantly improves hyperglycemia and hyperlipidemia in KKAy mice [16] and glucokinase mediates the expression of insulin receptor substrate 2 to alleviate islet -cell apoptosis caused by endoplasmic reticulum stress [17]. Figure S2: Colonization ability of Bifidobacterium longum 070103. Bifidobacterium longum is one of the most abundant species of probiotics in the gut microbiome in early years. Thus, the prior art describes probiotic approaches for achieving weight reduction, both of which elicit their effects at a post-prandial level without having any effect on food intake. EVC001 treatment promoted weight. In the human intervention study, no difference was observed in the primary outcome of BMI, even though the. Although no effect was observed on the basal level of -arrestin recruitment, a significant potentiation effect of ghrelin was observed when cells were first exposed to B. longum APC1472 supernatant (FIG. B Longum controls pH level of your gut: This beneficial bacteria helps lower & increase pH level of your gut, thereby maintaining a healthy gut. In one embodiment, the composition comprises a prebiotic material. The data were analyzed using Compound Discoverer 3.1 software (Thermo Fisher Scientific, Waltham, MA, USA) for baseline correction, peak differentiation, and alignment [27] and the result (CSV file) was exported to Microsoft Excel. provided funding and reviewed/edited the manuscript. A VIP > 1.5, p < 0.01, and FC > 2 was considered statistically significant. The invention may also employ growth media in which the strain of the invention was grown, or cell lysates generated using the strain of the invention. 16. [25] The contribution ratios of different metabolites to metabolic pathways are shown in Figure 5E. 3. Mechanisms regulating intestinal barrier integrity and its pathological implications. 5.. Sugar utilization gene clusters in B. infantis strains. contributed to the design of the preclinical study, performed the preclinical study and contributed to the writing of the manuscript of the preclinical study. Genetic modifications may be carried out using recombinant DNA techniques and reagents, including gene editing technologies such as CRISP-Cas9 techniques (see below). 4. [2] In addition, B. longum can uniquely ferment galactomannan-rich natural gum using glucosaminidases and alpha-mannosidases that participate in the fermentation of glucosamine and mannose, respectively. The composition may comprise an additional probiotic bacterium. Unable to load your collection due to an error, Unable to load your delegates due to an error, The H1-H5 gene clusters encode proteins that transport and metabolize HMOs and are characteristic features of. Macropunches of hypothalamus were taken for RNA expression analysis. Therefore, it is possible that the regulation of intestinal microbes leads to changes in metabolites, repairs the intestinal barrier, and improves the disorder of glucose and lipid metabolism [50]. 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